Fabrication of plasmonic nanopyramidal array as flexible SERS substrate for biosensing application

The proposed work aims to develop a sensitive surface-enhanced Raman spectroscopy (SERS) nano-biosensor. inverted nano pyramid array on silicon substrate fabricated using electron beam lithography (EBL) was utilised as master template and the mold later replicated via nanoimprinting process prepare gold-coated polymer nanopyramid three-dimensional (3D) SERS substrate. fast versatile replication (NIL) can produce nanopyramids in low-cost reproducible fashion. Also, fabrication protocol be easily upscale for large scale fabrication. intense electric field confinement at nanotips four edges of enhanced signal probe molecules, i.e., Rhodamine 6G with limit detection down 3.277 × 10−9 M achieved. This also underlines efficiency arrays spectral hemoglobin proteins low concentrations. enhancement mechanism further studied through electromagnetic simulation COMSOL Multiphysics. In addition, bending test experiments were performed understand effect flexibility response. could pave way development platforms hazardous biological chemical compounds ultra-low concentrations practical applications.